tunel detection solution (Beyotime)
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Beyotime
tunel detection solution
Tunel Detection Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2054 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tunel+detection+solution/DAPI+Staining+Solution/pmc12960656-441-14-17
Average 99 stars, based on 2054 article reviews
Tunel Detection Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2054 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tunel+detection+solution/DAPI+Staining+Solution/pmc12960656-441-14-17
Average 99 stars, based on 2054 article reviews
tunel detection solution - by Bioz Stars,
2026-10
99/100 stars
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Labeling:Article Title: miR-542-3p attenuates corticosterone-induced hippocampal neuronal damage in depressive mice by modulating PTEN/AKT/GSK3β/β-catenin pathway Article Snippet: The sections were then blocked with a mixed solution of 5% BSA and 0.5% Triton X-100 for 2 h. Rabbit anti-NeuN (ab177487, 1:1000, Abcam) was added and incubated overnight at 4 ∘ C, followed by a 2-h incubation with goat anti-rat IgG (GB21302, 1:500, Servicebio, Wuhan, China). .. Next, a 20 μg/mL proteinase K solution (without DNase) was applied for 30 min. Apoptotic cells were labeled with 50 μL of Article Title: Neutrophil extracellular traps aggravate uremic cardiomyopathy by inducing myocardial fibroblast pyroptosis. Article Snippet: Pathological changes in the sections were observed under a microscope (Leica, DMi3000 B). .. Proteinase K working solution (50 μL) was added to sections of mouse myocardial tissue at 37 °C for 30 min. After washing with PBS, 5 μL TdT enzyme, 45 μL fluorescent labeling solution, and 50 μL TUNEL Assay:Article Title: miR-542-3p attenuates corticosterone-induced hippocampal neuronal damage in depressive mice by modulating PTEN/AKT/GSK3β/β-catenin pathway Article Snippet: The sections were then blocked with a mixed solution of 5% BSA and 0.5% Triton X-100 for 2 h. Rabbit anti-NeuN (ab177487, 1:1000, Abcam) was added and incubated overnight at 4 ∘ C, followed by a 2-h incubation with goat anti-rat IgG (GB21302, 1:500, Servicebio, Wuhan, China). .. Next, a 20 μg/mL proteinase K solution (without DNase) was applied for 30 min. Apoptotic cells were labeled with 50 μL of Article Title: Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration. Article Snippet: .. Subsequently, 50 μL of Article Title: Gastrodia protects HT22 cells from damage caused by oxygen glucose deprivation and reperfusion through inhibiting ferroptosis. Article Snippet: .. HT22 cells were exposed to 4% paraformaldehyde (Solarbio, Beijing, China) on slides for 15 min, and subsequently permeabilized with drops of 0.3% Tritonx-100 (Sigma-Aldrich, St. Louis, MO, USA) for 10 min. Article Title: Neutrophil extracellular traps aggravate uremic cardiomyopathy by inducing myocardial fibroblast pyroptosis. Article Snippet: Pathological changes in the sections were observed under a microscope (Leica, DMi3000 B). .. Proteinase K working solution (50 μL) was added to sections of mouse myocardial tissue at 37 °C for 30 min. After washing with PBS, 5 μL TdT enzyme, 45 μL fluorescent labeling solution, and 50 μL Article Title: Astragaloside IV Alleviates Ulcerative Colitis Progression by Inhibiting WDR5-Mediated ENO1 H3K4me3 Modification. Article Snippet: Apoptosis levels were detected using the one- step TUNEL apoptosis assay kit (C1086, Beyotime). .. The cells were fixed with immunol staining fix solution (P0098- 100 mL, Beyotime) for 30 min, rinsed with PBS, and incubated with enhanced immunostaining permeabilization solution (P0097- 100 mL, Beyotime) for 5 min at room temperature and with Article Title: Mitochondrial complex I subunit NDUFS4 overexpression drives glioma progression by regulating mitochondrial function and COX5B Article Snippet: .. After 15-minute fixation in 4% paraformaldehyde and 5-minute Triton treatment, cells were exposed to Article Title: FOXD1-activated ANXA3 facilitates cisplatin resistance of lung cancer cells via promoting ANXA4 expression. Article Snippet: The expression of Annexin A3 (ANXA3) is thought to be associated with chemoresistance in lung cancer.. However, the underlying molecular mechanisms of ANXA3-mediated cisplatin (DDP) resistance in lung cancer still need to be further explored.. The levels of ANXA3, forkhead box D1 (FOXD1), and Annexin A4 (ANXA4) were examined by qRT-PCR or Western blot. Article Title: Mechanosensing antagonizes ethylene signaling to promote root gravitropism in rice Article Snippet: .. Root tips were immersed in PBS containing 0.3% Triton X-100 for 5 mins, washed with PBS, and incubated in the Staining:Article Title: miR-542-3p attenuates corticosterone-induced hippocampal neuronal damage in depressive mice by modulating PTEN/AKT/GSK3β/β-catenin pathway Article Snippet: The sections were then blocked with a mixed solution of 5% BSA and 0.5% Triton X-100 for 2 h. Rabbit anti-NeuN (ab177487, 1:1000, Abcam) was added and incubated overnight at 4 ∘ C, followed by a 2-h incubation with goat anti-rat IgG (GB21302, 1:500, Servicebio, Wuhan, China). .. Next, a 20 μg/mL proteinase K solution (without DNase) was applied for 30 min. Apoptotic cells were labeled with 50 μL of Article Title: Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration. Article Snippet: .. Subsequently, 50 μL of Article Title: Gastrodia protects HT22 cells from damage caused by oxygen glucose deprivation and reperfusion through inhibiting ferroptosis. Article Snippet: .. HT22 cells were exposed to 4% paraformaldehyde (Solarbio, Beijing, China) on slides for 15 min, and subsequently permeabilized with drops of 0.3% Tritonx-100 (Sigma-Aldrich, St. Louis, MO, USA) for 10 min. Article Title: Neutrophil extracellular traps aggravate uremic cardiomyopathy by inducing myocardial fibroblast pyroptosis. Article Snippet: Pathological changes in the sections were observed under a microscope (Leica, DMi3000 B). .. Proteinase K working solution (50 μL) was added to sections of mouse myocardial tissue at 37 °C for 30 min. After washing with PBS, 5 μL TdT enzyme, 45 μL fluorescent labeling solution, and 50 μL Article Title: Astragaloside IV Alleviates Ulcerative Colitis Progression by Inhibiting WDR5-Mediated ENO1 H3K4me3 Modification. Article Snippet: Apoptosis levels were detected using the one- step TUNEL apoptosis assay kit (C1086, Beyotime). .. The cells were fixed with immunol staining fix solution (P0098- 100 mL, Beyotime) for 30 min, rinsed with PBS, and incubated with enhanced immunostaining permeabilization solution (P0097- 100 mL, Beyotime) for 5 min at room temperature and with Article Title: Mitochondrial complex I subunit NDUFS4 overexpression drives glioma progression by regulating mitochondrial function and COX5B Article Snippet: .. After 15-minute fixation in 4% paraformaldehyde and 5-minute Triton treatment, cells were exposed to Incubation:Article Title: Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration. Article Snippet: .. Subsequently, 50 μL of Article Title: Astragaloside IV Alleviates Ulcerative Colitis Progression by Inhibiting WDR5-Mediated ENO1 H3K4me3 Modification. Article Snippet: Apoptosis levels were detected using the one- step TUNEL apoptosis assay kit (C1086, Beyotime). .. The cells were fixed with immunol staining fix solution (P0098- 100 mL, Beyotime) for 30 min, rinsed with PBS, and incubated with enhanced immunostaining permeabilization solution (P0097- 100 mL, Beyotime) for 5 min at room temperature and with Article Title: FOXD1-activated ANXA3 facilitates cisplatin resistance of lung cancer cells via promoting ANXA4 expression. Article Snippet: The expression of Annexin A3 (ANXA3) is thought to be associated with chemoresistance in lung cancer.. However, the underlying molecular mechanisms of ANXA3-mediated cisplatin (DDP) resistance in lung cancer still need to be further explored.. The levels of ANXA3, forkhead box D1 (FOXD1), and Annexin A4 (ANXA4) were examined by qRT-PCR or Western blot. Article Title: Mechanosensing antagonizes ethylene signaling to promote root gravitropism in rice Article Snippet: .. Root tips were immersed in PBS containing 0.3% Triton X-100 for 5 mins, washed with PBS, and incubated in the Fluorescence:Article Title: Neutrophil extracellular traps aggravate uremic cardiomyopathy by inducing myocardial fibroblast pyroptosis. Article Snippet: Pathological changes in the sections were observed under a microscope (Leica, DMi3000 B). .. Proteinase K working solution (50 μL) was added to sections of mouse myocardial tissue at 37 °C for 30 min. After washing with PBS, 5 μL TdT enzyme, 45 μL fluorescent labeling solution, and 50 μL Article Title: Mitochondrial complex I subunit NDUFS4 overexpression drives glioma progression by regulating mitochondrial function and COX5B Article Snippet: .. After 15-minute fixation in 4% paraformaldehyde and 5-minute Triton treatment, cells were exposed to Article Title: Mechanosensing antagonizes ethylene signaling to promote root gravitropism in rice Article Snippet: .. Root tips were immersed in PBS containing 0.3% Triton X-100 for 5 mins, washed with PBS, and incubated in the Microscopy:Article Title: Neutrophil extracellular traps aggravate uremic cardiomyopathy by inducing myocardial fibroblast pyroptosis. Article Snippet: Pathological changes in the sections were observed under a microscope (Leica, DMi3000 B). .. Proteinase K working solution (50 μL) was added to sections of mouse myocardial tissue at 37 °C for 30 min. After washing with PBS, 5 μL TdT enzyme, 45 μL fluorescent labeling solution, and 50 μL Article Title: Mitochondrial complex I subunit NDUFS4 overexpression drives glioma progression by regulating mitochondrial function and COX5B Article Snippet: .. After 15-minute fixation in 4% paraformaldehyde and 5-minute Triton treatment, cells were exposed to Immunostaining:Article Title: Astragaloside IV Alleviates Ulcerative Colitis Progression by Inhibiting WDR5-Mediated ENO1 H3K4me3 Modification. Article Snippet: Apoptosis levels were detected using the one- step TUNEL apoptosis assay kit (C1086, Beyotime). .. The cells were fixed with immunol staining fix solution (P0098- 100 mL, Beyotime) for 30 min, rinsed with PBS, and incubated with enhanced immunostaining permeabilization solution (P0097- 100 mL, Beyotime) for 5 min at room temperature and with Transfection:Article Title: FOXD1-activated ANXA3 facilitates cisplatin resistance of lung cancer cells via promoting ANXA4 expression. Article Snippet: The expression of Annexin A3 (ANXA3) is thought to be associated with chemoresistance in lung cancer.. However, the underlying molecular mechanisms of ANXA3-mediated cisplatin (DDP) resistance in lung cancer still need to be further explored.. The levels of ANXA3, forkhead box D1 (FOXD1), and Annexin A4 (ANXA4) were examined by qRT-PCR or Western blot. Laser-Scanning Microscopy:Article Title: Mechanosensing antagonizes ethylene signaling to promote root gravitropism in rice Article Snippet: .. Root tips were immersed in PBS containing 0.3% Triton X-100 for 5 mins, washed with PBS, and incubated in the |